ymfC [2018-10-30 13:01:41]

similar to transcriptional regulator ([SW|GntR family])

locus: BSU16810

pI: 6.40

mw: 27.75 kDa

protein length: 241 aa

gene length: 723 bp

function: unknown

product: transcriptional regulator ([SW|GntR family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

Gene

Coordinates: 1,754,785 → 1,755,510

The protein

Protein family

• [SW|GntR family] of transcription factors

Structure

• [PDB|2WVO] ([protein|6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5|NagR], corresponds to aa 83 ... 307 of YmfC, 30% identity)

Expression and Regulation

Operons

• genes: [gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]
  description: [pubmed|22383849]

• genes: [gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]-[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507870], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B115 (ymfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1114 NBRP B. subtilis, Japan]
• BKE16810 (Δ[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCTCCGTAAAC, downstream forward: _UP4_TAAAAACGGACGCCTGTATG
• BKK16810 (Δ[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCCTCCGTAAAC, downstream forward: _UP4_TAAAAACGGACGCCTGTATG

References

• 23504016